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Islandora
Dissertations, Theses, and Projects
Undergraduate Honors Theses
2018 Honors Theses (Spring)
The Roles of glutathione s-transferase in the response of ovarian cancer cells to resveratrol and doxorubicin chemotherapy.
The Roles of glutathione s-transferase in the response of ovarian cancer cells to resveratrol and doxorubicin chemotherapy.
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Title
The Roles of glutathione s-transferase in the response of ovarian cancer cells to resveratrol and doxorubicin chemotherapy.
Creator
Subject
Cancer--Chemotherapy.
Description
Ovarian cancer cells have the ability to become resistant to different chemotherapy drugs, including one of the most commonly prescribed drugs Doxorubicin. This drug specifically is focused on during our research. Many previous research studies have investigated causes of ovarian cancer cell resistance, such as the protective enzyme glutathione S-transferase (GST). Studies have also been performed on methods of inhibiting this resistance, possibly through the use of resveratrol, a component found in grapes and red wine. However, some doses of resveratrol have been found to result in an increase in resistance. In order to further investigate the cell’s sensitivity or resistance, this experiment examined a range of doses of doxorubicin and resveratrol administered to ovarian cancer cells. The samples were later analyzed to see if they were preventing or promoting chemotherapy resistance. Doxorubicin and resveratrol pretreatment were administered to triplicate cultures of ovarian cancer cells, either together or separately, and were left in this pretreatment for two days. At this time, replicate dishes of the cells were tested for their GST activity. The GST activity data was collected from a spectrophotometer, measuring the amount of product that was produced via a colorimeteric reaction. We recognized that GST activity may be proportional to doxorubicin resistance in these pretreated ovarian cancer cells. Data was taken for control groups, resveratrol alone, doxorubicin alone, and a combination of doxorubicin and resveratrol treatments at various dosages. Questions aimed to be answered during this study were as follows: In cases where doxorubicin pretreatment results in resistance to doxorubicin, is it accompanied with increased GST levels or are they unrelated. Additionally, if resveratrol causes resistance to doxorubicin alone does it do so by increasing GST levels or through an alternative mechanism. In other words, can the varying degrees of doxorubicin resistance be caused by doxorubicin and/or resveratrol pretreatment. Also, will doxorubicin resistance reflect an increase in GST activity (i.e. low GST activity relays low resistance, high GST activity relays high resistance). Further studies will be needed to confirm if resistance was formed by the ovarian cancer cells due to receiving the resveratrol and doxorubicin pretreatments, Honors Thesis Advisor : Dr. Paula Caffrey.
Publisher
Contributor
Berry, Emily A. (Author)
Date
2018-05-02
Type
Text
Format
Identifier
cali:770
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