Examining the Transgenerational Effect of TCDD on Bone Degradation in Zebrafish

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Title: Examining the Transgenerational Effect of TCDD on Bone Degradation in Zebrafish
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Description: Agent Orange is a chemical herbicide composed of the two chemicals 2,4-dichlorophenoxyacetic acid (2,4-d) and 2,4,5-trichlorophenoxyacetic acid (2,4,5-t). During the mass production of the herbicide at the height of the Vietnam War over 12 million gallons of contaminated Agent Orange was sprayed on the affected area. Agent orange produces a highly toxic byproduct 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) or dioxin which has been known to cause a variety of gastrointestinal, respiratory, and systematic problems, including a variety of cancers. This highly toxic chemical is now being studied to understand the scope of dioxin-related illnesses. Interestingly, dioxin research has shown that the chemical can devastatingly impact the composition and quality of bone. In this research project, adult and embryonic Zebrafish were exposed to varying concentrations of TCDD and observed throughout breeding and post-exposure stages to understand the effect TCDD has on first generation-exposure offspring. Alizarin red was used to detennine whether fish developing from embryos exposed to TCDD or fish developing from embryos where parents had been exposed to TCDD showed structural changes in bone compared to controls. DNA methylation levels are being quantified to indicate whether exposed embryos and/or offspring show specific structural and molecular changes within the genome that might indicate that TCDD exposure causes epigenetic changes., Honors Thesis Advisor : Dr. Louise Nicholson, PhD.
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Contributor: Davis, Savannah L. (Author), California University of Pennsylvania. Honors Program.
Date: 2022-04-20
Type: Text
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Identifier: cali:2253
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